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Expression of KCC2 phospho-mutants in HEK-293 cells. KCC2WT and the indicated KCC2 mutants were expressed in HEK-293 cells and efficiently delivered to the plasma membrane. The nucleus was devoid of staining. Upon visual inspection, the subcellular distribution of KCC2 mutants was indistinguishable from KCC2WT. Photomicrographs were taken with a confocal laser scanning microscope. Scale bar, 10 μm.
