TABLE 4.

Transport activity of mutant KCC2^Thr-934 and KCC2^Ser-937 in the presence of NEM or staurosporine

	Basal conditions (significance in comparison with KCC2WT)	1 mm NEM (significance in comparison with untreated samples or KCC2WT under basal conditions)	8 μm staurosporine (significance in comparison to the untreated samples) (or KCC2WT under basal conditions)
HEK-293	8.3 ± 1.9%a	19.2 ± 2.2%b	24.8 ± 4.4%b
KCC2WT	100 ± 4.9%	157 ± 8.2%b	180.7 ± 11.5%b
T934A	97.3 ± 2.1% (NS)c	159.3 ± 13%b	199.5 ± 8.3%d
T934D	226.6 ± 13%a	86 ± 4.1%a (NS)	123 ± 12.6%a (NS)
S937A	106.2 ± 10.2% (NS)	159.6 ± 9.3%d	199.5% ± 16.7%b
S937D	186.9 ± 12%a	100 ± 8.2%a (NS)	132.1 ± 8.5%d (NS)
T934A/S937A	119.4 ± 9.8% (NS)	148.2 ± 3.2%b	198% ± 5.4%a

^a p < 0.001.

^b p < 0.05.

^c NS, not significant.

^d p < 0.01.