FIGURE 1.

Effect of THC on lymph node cell proliferation and Th1 and Th2 subpopulations. C57BL/6J mice were treated with THC or vehicle as described under “Experimental Procedures” on days 0 and 1, and 2 h later, 10 μg of SEB was injected into each foot pad. Three days after SEB challenge, draining popliteal lymph nodes of SEB + vehicle- or SEB + THC-treated mice (n = 3) were harvested, and cells were analyzed. a, total cells in two popliteal lymph nodes in each mouse. b, cells were gated by CD4⁺ and analyzed by FACS for the expression of Ki67. c–f, based on flow cytometric analysis as described under “Experimental Procedures,” cell numbers of various CD4⁺ T cell subpopulations expressing IFN-γ, TBX21, IL-4, or GATA3 were depicted. g and h, overall frequency and mean fluorescence intensity (MFI) of Ki67, CD4, and IFN-γ triple-positive cells. i and j, overall frequency and MFI of Ki67, CD4, and IL-4 triple-positive cells. p values were determined by Student's t test. Error bars, S.E.