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Purified wild-type UppP and mutants. The lanes from left to right are as follows: wild-type UppP (lanes 1 and 19); E17A (lane 2); E21A (lane 3); E17A-E21A (lane 4); H30A (lane 5); E41A (lane 6); D43A (lane 7); E49A (lane 8); Q53A (lane 9); D111A (lane 10); D150A (lane 11); S173A (lane 12); R174A (lane 13); S175A (lane 14); T178A (lane 15); R189A (lane 16); E194A (lane 17), and R261A (lane 18).
