FIGURE 5.

Enzymatic activity analysis. A, effect of pH on UppP activity was assayed at various pH values, pH 5.0–6.0 (sodium acetate), pH 6.5–8.0 (Hepes), and pH 9 (Tris-HCl). B, effect of the detergent concentration on the phosphatase activity. The concentration of DDM was utilized in the range of 0.02 to 1%. C, effect of the EDTA on the UppP phosphatase activity. D, metal ion requirements. The experiment was carried out by incubating 20 nm enzyme and various divalent cations (Mg²⁺, Mn²⁺, Zn²⁺, Co²⁺, and Ca²⁺) at a concentration of 10 mm. Control indicates sample without addition of extra metal ions or EDTA.