|
|
% |
|
Region I |
|
|
|
E17A |
Active site (P-site) |
26 |
H-bond with pyrophosphate of UppP via the Mg2+
|
E21A |
Active site (P-site) |
40 |
H-bond with pyrophosphate of UppP via the Mg2+
|
E17A/E21A |
Active site (P-site) |
<1 |
H-bond with pyrophosphate of UppP via the Mg2+
|
H30A |
Active site (P-site) |
<1 |
Initiating a nucleophilic attack on the phosphorus center |
|
Region II (structural P-loop) |
|
|
|
S173A |
Active site (P-site) |
<1 |
The backbone oxygen H-bond with Arg-174 |
R174A |
Active site (P-site) |
<1 |
H-bond with oxygen atom of α-phosphate of UppP |
S175A |
Active site (P-site) |
32 |
Interaction with phosphate group of UppP |
T178A |
Active site (P-site) |
<1 |
H-bond with the backbone NH of Arg-174 |
|
Other mutants |
|
|
|
E41A |
Aqueous interface (P-site) |
85 |
Loop structural maintenance |
D43A |
Aqueous interface (P-site) |
73 |
Loop structural maintenance |
E49A |
Membrane-water interface (P-site) |
36 |
TM2 structural maintenance |
Q53A |
Membrane-water interface (P-site) |
14 |
TM2 structural maintenance |
D111A |
Membrane-water interface (P-site) |
100 |
|
E137A |
At the end of TM4 (C-site) |
ND |
Salt bridge with Arg-67 and His-94 |
D150A |
Membrane-water interface (C-site) |
64 |
|
R189A |
Aqueous interface (P-site) |
11 |
Stabilizing His-30 |
E194A |
Aqueous interface (P-site) |
31 |
Stabilizing Arg-189 |
R261A |
At the end of TM8 (P-site) |
<1 |
H-bond with Ser-173 |