FIGURE 4.

Effect of temperature and incubation time on the stability of NDH-1L and NDH-1M complexes from the WT and ΔndhP strains. A and D, profiles of BN-PAGE of the thylakoid membranes isolated from the WT and ΔndhP strains. The membranes were incubated with 1.5% DM on ice for 60 min (at 25 °C for 0 min) or at 25 °C for 10, 20, 30, and 60 min. Western analyses of NDH-1L (red arrows) and NDH-1M (blue arrows) of the WT (B and C) and ΔndhP (E and F) isolated in A and D, using antibodies against NdhK and NdhM. Asterisk represents nonspecific signals.