FIGURE 8.

Effect of temperature and incubation time on the stability of NDH-1L and NDH-1M complexes from the WT-NdhP-YFP-His₆ strain. A, profiles of BN-PAGE of thylakoid membranes isolated from the WT-NdhP-YFP-His₆ strain treated with 1.5% DM on ice for 60 min (at 25 °C for 0 min) or at 25 °C for 10, 20, 30, and 60 min. Red and blue arrows indicate the positions of NDH-1L and NDH-1M, respectively. A band with the apparent molecular mass of about 160 kDa is shown by a green arrow. B–D, Western analyses of protein complexes separated by BN-PAGE using the antibodies against NdhK (B), NdhM (C), and GFP (D). Asterisk represents nonspecific signals.