FIGURE 9.
Yeast two-hybrid system for assaying the interaction of NdhP with NdhD1, NdhF1, and NdhB. ndhP, ndhPN+TM, and ndhPC were constructed into bait vector, whereas ndhD1, ndhF1, and ndhB were contracted into prey vector, respectively. Subsequently, they were transformed into the yeast strain EGY48. Transformed yeast was dropped onto X-gal medium. Blue precipitate represents accumulated β-galactosidase activity resulting from the activation of the lacZ reporter gene by protein-protein interaction. The induction plate was incubated at 30 °C for 28 h and then photographed. The interaction of NdhA with NdhF1 was assayed as a negative control. At least six independent experiments were performed, and one representative result is shown on each assay.