TABLE 4.
SNO actin/β-actin band density ratios from air- and 690-kPa N2-exposed neutrophils
Data reflect band densities on Western blots of lysates from cells exposed to air or air + 690 kPa of N2 for 30 min that had been subjected to the biotin switch procedure. PBS, cells incubated with buffer only during air/N2 exposure; UV, cells exposed to UV light for 5 min after air/N2 exposure. Other interventions involved incubation with 5 μm cytochalasin D, 1 mm 1400W, or 10 μm Nox2ds during air/N2 exposure. All values are mean ± S.E. (n = number of independent trials (shown in parentheses)).
| Incubation/intervention | Air | N2 |
|---|---|---|
| PBS | 1.00 ± 0.00 (5) | 2.50 ± 0.34 (5)a |
| UV | 1.03 ± 0.11 (3) | 1.07 ± 0.17 (3) |
| Cytochalasin D | 1.22 ± 0.04 (3) | 1.10 ± 0.05 (3) |
| Nox2ds | 0.99 ± 0.09 (3) | 1.08 ± 0.14 (3) |
| 1400W | 1.04 ± 0.04 (3) | 1.08 ± 0.09 (3) |
a p < 0.05 versus control.