TABLE 5.

Protein associations assessed by DTSP cross-linking in sF-actin, G-actin, and Triton-insoluble actin fractions of neutrophils exposed to air (control) or for 30 min to 690 kPa of N₂

Values are ratios calculated based on the band densities of the identified protein relative to the actin band density in each sample. In each section, the values are normalized to air-exposed control protein band ratios of individual experiments; thus, values greater than 1.0 reflect increases in protein associations with actin, whereas values less than 1.0 indicate lower protein associations. Where indicated, cells were exposed to UV light for 5 min prior to the addition of DTSP to cross-link proteins and then fractioned based on Triton solubility (see “Experimental Procedures”). Data are mean ± S.E. (n = 5 for all samples).

	VASP/actin	FAK/actin	iNOS/actin	Flip/actin	Flop/actin	PDI/actin
sF-actin
690 kPa of N2	4.68 ± 1.21a	2.91 ± 0.39a	2.79 ± 0.29a	2.48 ± 0.45a	3.28 ± 0.68a	4.03 ± 0.43a
Air + UV	1.10 ± 0.11	1.09 ± 0.08	1.22 ± 0.08	1.02 ± 0.07	1.19 ± 0.15	1.11 ± 0.04
N2 +UV	0.86 ± 0.11	0.96 ± 0.08	1.38 ± 0.37	1.01 ± 0.15	1.17 ± 0.26	0.88 ± 0.11
G-actin
690 kPa of N2	0.12 ± 0.05a	0.19 ± 0.05a	0.27 ± 0.08a	0.17 ± 0.06a	0.45 ± 0.13a	0.36 ± 0.08a
Air + UV	0.92 ± 0.05	0.99 ± 0.09	1.58 ± 0.43	0.87 ± 0.02	1.03 ± 0.22	1.08 ± 0.13
N2 +UV	0.89 ± 0.16	1.13 ± 0.19	1.45 ± 0.29	0.81 ± 0.14	1.07 ± 0.15	1.33 ± 0.24
Triton-insoluble
690 kPa of N2	0.16 ± 0.06a	0.19 ± 0.06a	0.22 ± 0.10a	0.29 ± 0.09a	0.35 ± 0.13a	0.20 ± 0.03a
Air+ UV	1.15 ± 0.22	1.14 ± 0.10	1.46 ± 0.27	1.56 ± 0.30	0.97 ± 0.01	0.96 ± 0.06
N2 +UV	0.916 ± 0.20	0.93 ± 0.17	0.94 ± 0.18	1.32 ± 0.22	1.03 ± 0.15	0.75 ± 0.13

^a Values significantly different from normalized air-exposed control values (p < 0.05, ANOVA).