TABLE 6.

Immunoprecipitation of short F-actin fraction from lysates of murine neutrophils exposed to 690 kPa of N₂ for 30 min versus those incubated in air (set as a protein/actin ratio = 1 for each experiment)

The short F-actin fraction was isolated from neutrophils and subjected to immunoprecipitation using antibodies to actin. Where indicated, cells were exposed to UV light for 5 min after air/N₂ incubation and prior to lysis. Western blots were carried out, and data are expressed as the ratio of protein band densities versus actin band density for each lysate normalized to the ratio found with air-exposed (control) cells in each experiment; thus, values are normalized to 1.0 for air-exposed cells without UV exposure. Data are mean ± S.E. (n = 6).

	VASP/actin	FAK/actin	iNOS/actin	Flip/actin	Flop/actin	PDI/actin
690 kPa of N2	1.61 ± 0.24a	2.15 ± 0.20a	1.57 ± 0.34a	1.38 ± 0.21	1.36 ± 0.41	0.99 ± 0.11
N2 + UV	0.88 ± 0.20	1.11 ± 0.14	1.19 ± 0.24	0.85 ± 0.20	1.49 ± 0.43	0.84 ± 0.09
Control/UV	1.17 ± 0.17	1.21 ± 0.19	1.13 ± 0.27	1.02 ± 0.11	0.96 ± 0.24	1.26 ± 0.11

^a p < 0.05 ANOVA.