FIGURE 1.

Altered extractability and electrophoretic pattern of Fmod^−/− tail tendon collagen. A, collagen yields on sequential extraction (PBS, 0.5 m acetic acid, and pepsin) of tail tendons from Fmod^−/− and wild-type mice. Filled bars, wild-type mice (n = 5); open bars, Fmod^−/− mice (n = 5). B, 8% SDS-PAGE of acetic acid-extracted collagen from wild-type (n = 3) and Fmod^−/− (n = 3) mice stained with Coomassie Blue. Relative amounts of α-, β-, and γ-components of collagen were quantified with ImageJ software (National Institutes of Health) and are listed as percentages of total collagen ± S.D.