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. 2014 Apr 30;289(27):18987–18998. doi: 10.1074/jbc.M114.557686

FIGURE 4.

FIGURE 4.

HSP70 regulates NOD2 level in the cell. A, cell extracts from HSP70 or control vector-transfected HEK293T/NOD2Myc cells were probed using rabbit anti-Myc antibody (1:1000) and rabbit anti-HSP70 antibody (1:1000). β-Actin (1:1000) was used as a loading control. B, cell extracts of HEK293T-NOD2-Myc/Tet-op cells incubated with 100 μm of GGA or DMSO were probed using rabbit anti-Myc antibody (1:1000) and rabbit anti-HSP70 antibody (1:1000). β-Actin (1:1000) was used as a loading control. C, cell extracts of HEK293T-NOD2-Myc/Tet-op cells incubated with 100 μm KNK437 or DMSO was probed using rabbit anti-Myc antibody (1:1000) and rabbit anti-HSP70 antibody (1:1000). β-Actin (1:1000) was used as a loading control. D, cell extracts of HEK293T-NOD2-Myc/Tet-op cells incubated with 25, 50, or 100 μm of KNK437 or DMSO were probed using rabbit anti-Myc antibody (1:1000). β-Actin (1:1000) was used as a loading control. Western blots were performed on separate cell lysates at least three times. Using the replicates, the bands were quantified using ImageLabTM.