FIGURE 13.

Rapamycin sensitivity or resistance of Gln3 mutant proteins. Serial dilutions (1:10) were made of gln3Δ (KHC2) transformants containing the plasmids listed on the left side of the figure. All transformants were grown on SC medium without uracil. Control plates devoid of rapamycin (left column of images) were photographed after about 3 days of incubation at 30 °C, whereas those containing 50 ng/ml rapamycin (right column of images) were incubated for about 5 days. The pertinent genotype or types of substitutions made in the mutant proteins are listed to the right of the images. W.T. indicates wild type, and vector indicates a vector-negative control that is devoid of Gln3. The panels depict individual plates. The presence of some strains (e.g. pRR536 or pRR614) in more than one panel permits comparisons between plates. A fine white line between two panels indicates that the two panels came from different locations on the same plate. Detailed descriptions of some mutants used in this experiment appear in a previous paper (40). They are pRR614 (Gln3(1–600)-Myc¹³), pRR850 (Gln3_{S656D,S659D,S662D}-Myc¹³), pRR1038 (Gln3_{S656A,S659A,S662A}-Myc¹³), pRR922 (Gln3_S616D,_S617D,_{S619D,S621D,S624D,S631D}-Myc¹³), and pRR960 (Gln3_{S616A,S617A,S619A,S621A,S624A,S631A}-Myc¹³).