FIGURE 2.

Localization of full-length Gln3(1–730)-Myc¹³ (pRR536), Gln3_{N510D,N513D,Q515D,Q516D}-Myc¹³ (pRR1115), and Gln3_N510D,N513D-Myc¹³ (pRR1171) in untreated glutamine-grown (Gln in A–D), proline-grown (Pro in C and D), and ammonia-grown (Am. in E and F) transformants or in those treated with rapamycin (+Rap in A and B) or Msx (+Msx in E and F). A, C, and E depict representative images from which the corresponding histograms in B, D, and F were generated. Red bars indicate Gln3-Myc¹³ staining in the cytoplasm only; yellow bars indicate both cytoplasmic and nuclear (Nucl.-Cyto.) staining, and green bars indicate staining in the nucleus only. The nature of the amino acid substitution(s), i.e. aspartate, alanine, or none (WT) appears below the plasmid numbers. Strain JK9-3da was the transformation recipient.