FIGURE 2.

KIC- and glucose-stimulated OCR by islets cultured in RPMI 1640 medium containing either 11 mm or 3 mm glucose for 40 h. Islets were perifused in the presence of 3 mm glucose for 90 min. Subsequently, 10 mm KIC was added and removed, and 20 mm glucose and nimodipine were added to and removed from the inflow at the times shown on the graph. *, p < 0.05; **, p <0.01. Statistics were also calculated comparing islets cultured at 3 and 11 mm glucose. Difference in response to KIC is nonsignificant; responses to 20 mm glucose and nimodipine were different for the two culture conditions (p < 0.05).