FIGURE 11.

Regulation of pro-inflammatory gene expression in THP1 cells by glycosylated and nonglycosylated forms of flagellin. THP1 cells were stimulated for 24 h in the absence (NT, nontreated) or presence of varying concentrations of fully glycosylated wild-type (WT) or nonglycosylated (Δ0111) forms of flagellin, purified from the B. cenocepacia parental or ΔBCAL0111 strains, respectively. Conditioned media were assayed for expression levels of IL-1β (A), TNF-α (B), and IL-6 (C). Data are representative of three independent experiments. Statistical analysis was performed by paired t test using two-tailed p values. Significant differences between samples from WT and Δ0111-treated cells are indicated by * (p < 0.05), ** (p < 0.01), or ***, (p < 0.001).