TABLE 3.
Peptide ions identified after combining MS/MS data from tryptic, chymotryptic, and AspN/tryptic digests of B. cenocepacia FliC
Representative unmodified and modified ions are presented. Ions were confirmed in QTof and Orbitrap Elite analyses; (+231) refers to glycan modification Qui4N(3HOBut); oxidation refers to methionine (+16 Da).
| Start-End |
Mr |
Oxidation (+16) | Sequence | ||
|---|---|---|---|---|---|
| Observed | Calculated | Expected | |||
| m/z | |||||
| 1–36 | 954.2421 | 3812.9393 | 3812.9915 | Yes | MLGINSNINSLVAQQNLNGSQNALSQAITRLSSGKR |
| 37–52 | 773.3992 | 1544.7838 | 1544.7794 | No | INSAADDAAGLAISTR |
| 53–90 | 992.7329 | 3966.9025 | 3966.9011 | Yes (2 x) | MQTQINGLNQGVSNANDGVSMIQTASSALSSLTNSLQR |
| 91–106 | 840.9565 | 1679.8984 | 1679.8512 | Yes | IRQLAVQASTGTMSTT |
| 107–137 | 1154.2551 | 3459.7435 | 3459.7230 | No | DQAALQQEVSQQIQEVNRIASQTTYNGTNIL |
| 138–158 | 1010.5225 | 2019.0304 | 2019.0273 | No | DGSAGIVSFQVGANVGQTISL |
| 159–168 | 519.2255 | 1036.4364 | 1036.485 | No | DLSQSMSAAK |
| 159–168 | 527.2358 | 1052.4570 | 1052.4808 | Yes | DLSQSMSAAK |
| 159–168 | 642.8051 | 1283.5956 | 1283.5677 | Yes | DLSQSMSAAK (+231)a |
| 169–176 | 386.2400 | 770.4654 | 770.4650 | No | IGGGLVQK |
| 177–211 | 1118.2345 | 3351.6817 | 3351.6794 | No | GQTVGTVTGLSLDNNGAYTGSGATITAINVLSDGK |
| 177–211 | 1195.2588 | 3582.7546 | 3582.7663 | No | GQTVGTVTGLSLDNNGAYTGSGATITAINVLSDGK (+231) |
| 187–206 | 1085.5468 | 2169.0790 | 2169.0199 | No | SLDNNGAYTGSGATITAINV (+231) |
| 187–211 | 813.7294 | 2438.1664 | 2438.1925 | No | SLDNNGAYTGSGATITAINVLSDGK |
| 187–211 | 890.7588 | 2669.2546 | 2669.2794 | No | SLDNNGAYTGSGATITAINVLSDGK (+231) |
| 189–208 | 969.9615 | 1937.9084 | 1937.9330 | No | DNNGAYTGSGATITAINVLS |
| 189–208 | 724.0175 | 2169.0307 | 2169.0199 | No | DNNGAYTGSGATITAINVLS (+231) |
| 189–217 | 1032.8304 | 3095.4694 | 3095.4333 | No | DNNGAYTGSGATITAINVLSDGKGGYTFT (+231) |
| 212–233 | 904.7971 | 2709.2692 | 2709.2659 | No | GGYTFTDQNGGAISQTVAQSVF (2× 231)b |
| 234–251 | 1055.0292 | 2108.0438 | 2108.0009 | No | GANATTGTGTAVGNLTLQ (2× 231)a |
| 252–286 | 1258.6530 | 3772.9372 | 3772.9331 | No | SGATGAGTSAAQQTAITNAIAQINAVNKPATVSNL (2× 231) |
| 287–321 | 1176.9285 | 3527.7637 | 3527.7637 | Yes | DISTVSGANVAMVSIDNALQTVNNVQAALGAAQNR |
| 287–321 | 1253.9667 | 3758.8745 | 3758.8745 | Yes | DISTVSGANVAMVSIDNALQTVNNVQAALGAAQNR (+231) |
| 290–321 | 1148.9210 | 3443.7412 | 3443.7076 | Yes | TVSGANVAMVSIDNALQTVNNVQAALGAAQNR (+231) |
| 322–357 | 952.9465 | 3807.7569 | 3807.7381 | Yes | FTAIATSQQAESTDLSSAQSQITDANFAQETANMSK |
| 359–382 | 849.4679 | 2545.3819 | 2545.4592 | No | QVLQQAGISVLAQANSLPQQVLKL |
| 371–384 | 790.4595 | 1578.9044 | 1578.9093 | No | QANSLPQQVLKLLQ |
a Data were obtained with QTof only.
b Data were obtained with Orbitrap Elite only.