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. 2014 Jul 1;20(1):41–53. doi: 10.1016/j.cmet.2014.03.031

Figure 6.

Figure 6

Effect of Activation of Hypothalamic AMPK on the Central Actions of E2 on Energy Balance

(A) Representative immunofluorescence (20×) with anti-GFP antibody showing GFP expression in the VMH of OVX rats treated with adenoviruses encoding AMPKα-CA within the VMH.

(B–D) (B) Body weight change, (C) daily food intake, and (D) mRNA expression profile in the BAT (left panel) and core temperature (at 24 hr, right panel) of OVX rats SC treated with vehicle or E2 (VMH in [D], right) and stereotaxically treated with GFP or AMPKα-CA adenoviruses within the VMH.

(E) Representative immunofluorescence (20×, left panel, and 40×, right panel) with anti-GFP antibody showing GFP expression in the ARC of rats treated with adenoviruses encoding AMPKα-CA within the ARC.

(F–H) (F) Body weight change, (G) daily food intake, and (H) mRNA expression profile in the BAT of OVX rats SC treated with vehicle or E2 and stereotaxically treated with GFP or AMPKα-CA adenoviruses within the ARC. Error bars represent SEM; n = 8–17 animals per experimental group. 3V: third ventricle; ∗, ∗∗, and ∗∗∗p < 0.05 and 0.01 vs. vehicle GFP VMH or ARC; #, ## and ### p < 0.05 and 0.01versus E2 GFP VMH or ARC.