Fig 2.

The immunohistochemical staining of CRBN in myeloma cells. Aggregated myeloma cells highlighted by CD138 membranous staining in (a) and (b) (×400). Positive CRBN cytoplasmic/nuclear staining in myeloma cells with the same slice as in a is shown in (c) (×400). Negative CRBN staining in myeloma cells with the same slice as in b is shown in (d) (×400). Another positive CRBN cytoplasmic staining in immunoblastic-like myeloma cells (e) (×400) and a higher magnification (×1000, oil lens) for the cellular details of granular cytoplasmic pattern is inserted. Aggregates of myeloma cells with intense cytoplasmic staining for CRBN and less distinct nuclei (f) (×1000, oil lens). Typical myeloma cells stained positive for CRBN (arrow) were shown in (g) and (h) (×1000, oil lens). Several CRBN negative myeloid and mononuclear cells were noted in g (arrow head) and a cluster of CRBN negative erythrocytes are noted in h (arrow head)