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. 2014 Mar 13;23(15):4015–4023. doi: 10.1093/hmg/ddu115

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© The Author 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Expression and purification of METTL23 in E. coli in the absence and presence of chaperone coexpression. (A)The expression of METTL23 in the absence of chaperone coexpression in E. coli. METTL23 was found solely in inclusion bodies (left lane), whereas the supernatant was devoid of METTL23 (right lane). (B) The purification of METTL23 coexpressed with GroEL-GroES by Ni-NTA affinity and size exclusion chromatography. The middle lane was supernatant of METTL23 coexpressed with GroEL-GroES chaperones after cell lysis. The right lane is a fraction of METTL23 after Ni-NTA affinity and size exclusion chromatography.