Skip to main content
. 2014 Mar 13;23(15):4024–4034. doi: 10.1093/hmg/ddu116

Figure 6.

Figure 6.

Removing the β2ARs restores PKA levels in the P301S mice. (A) Representative western blots of proteins extracted from the brains of CTL, P301S and β2KO/P301S mice and probed with the indicated antibodies. (B and C) Quantitative analyses of the total and phospho-AKT blots show that total AKT levels were significantly reduced in the P301S mice compared with WT and β2KO/P301S mice. In contrast, the levels of AKT phosphorylated at Ser473 were similar among the three groups. (D and E) Quantitative analyses of the p54 and p46 JNK isoforms show that the levels of the p54 isoform were similar among the three groups, while p46 levels were significantly lower in CTL mice compared with the other two groups. (F and G) Quantitative analyses of the levels of the PKA regulatory subunit IIα indicate that the levels of the low molecular weight band of PKA were significantly different in the brains of P301S mice compared with the other two groups. Quantifications of the western blots were performed by normalizing the protein of interest to β-actin, which was used as a loading control. n = 6/genotype. Data are presented as means ± SEM and analyzed by one-way ANOVA. * indicates P < 0.05.