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. 2014 Mar 25;23(15):4077–4085. doi: 10.1093/hmg/ddu121

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© The Author 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — PNPLA3 is highly expressed in human retina, hepatocytes and HSCs. (A) PNPLA3 mRNA expression in human tissues assessed by qPCR. The tissue with the highest CT value was assigned the value of 1. (B) PNPLA3 mRNA expression in pHSCs, primary human hepatocytes (positive control) and human colon carcinoma (CACO-2) cells (negative control). Lecithin retinol acyl transferase (LRAT) and albumin were used as references for pHSCs and hepatocytes, respectively. (C) Immunoblot showing PNPLA3 and PNPLA2 protein expression in pHSCs, primary human hepatocytes (Hep) and CACO-2 cells. Calnexin was used as a loading control.