FRMD7 mRNA expression profile in developing human neural retina. (A) Low-magnification image of the embryos from CS 15, 16, 19, 23 and 9 weeks postconception (wpc). (B) Dynamic expression pattern; expression initially confined to the outer neuroblastic layer (ONBL) at CS15, subsequently expression seen within the inner neuroblastic layer (INBL). Bilaminar expression pattern at 9 wpc (arrows). Expression within the developing optic stalk (OPS) at CS15, CS16 and CS19. Expression restricted to the optic nerve sheath (ONS), absent in developing optic disk (OD) at 9 wpc. Peripheral neural retina is the last to differentiate and laminate, hence differential expression between central and peripheral neural retina, most evident at CS16 and CS19. Sense images shown below the antisense images; once pigmentation occurs, RPE appears as false positive for expression at CS16 onward. Low-magnification image scale bar: 500 μm high-magnification image scale bar: 200 μm.