Table 1. Dissociation Rate Constants Measured for Various Drugs with Soluble HSA by Using Ultrafast Affinity Extraction on HSA Microcolumnsa.
| dissociation rate constant (kd (s–1)) | |||
|---|---|---|---|
| drug | estimate (eq 4b) | estimate (eq 5) | literature [ref] |
| warfarin | 0.80 (±0.05) | 0.72 (±0.05) | 0.41–2 [9, 15, 40] |
| tolbutamide | 0.59 (±0.03) | 0.58 (±0.04) | 0.49 (±0.15) [15] |
| acetohexamide | 0.67 (±0.03) | 0.63 (±0.03) | 0.58 (±0.02) [15] |
| verapamil | 0.35 (±0.02) | 0.36 (±0.02) | 0.38 (±0.05) [17] |
| gliclazide | 0.61 (±0.02) | 0.59 (±0.04) | not reported |
| chlorpromazine | 3.96 (±0.13) | 3.35 (±0.30) | not reported |
a
The kd values were measured at pH 7.4 and at 37 °C. Each of the injected samples contained 10 μM of the drug and 20 μM of HSA. The values in the parentheses represent a range of ±1 SD, as determined from the slopes of the best-fit lines constructed according to eqs 4 and 5.
b
These values were found by using eq 4 when a point at the origin was included in the data set.