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. 2014 Jun 9;86(13):6454–6460. doi: 10.1021/ac501031y

Table 2. Equilibrium Constants Measured for Various Drugs with Soluble HSA by Using Ultrafast Affinity Extraction on HSA Microcolumnsa.

association equilibrium constant, Ka, or global affinity constant, nKa′ (M–1)
drug estimate (eq 6) estimate (eqs 5 and 6) literature [ref]
warfarin 2.4 (±0.4) × 105 1.6 (±0.2) × 105 2.0–5.7 × 105 [182930]
tolbutamide 1.1 (±0.4) × 105 0.9 (±0.2) × 105 1.1 (±0.1) × 105 [19]b
acetohexamide 1.8 (±0.5) × 105 1.3 (±0.1) × 105 1.7(±0.1) × 105 [20]b
verapamil 1.5 (±0.4) × 104 1.6 (±0.2) × 104 1.4 (±0.1) × 104 [21]c
gliclazide 8.0 (±0.6) × 104 6.9 (±1.0) × 104 7.9 (±0.1) × 104 [22]b
chlorpromazine 6.2 (±0.5) × 104 4.9 (±0.5) × 104 6.4 × 104 [23]
a

These results were measured at pH 7.4 and at 37 °C. The values in parentheses represent a range of ±1 S.D., as determined by error propagation.

b

The global affinity constants for these drugs were calculated from data in the given references.

c

This value represents the average association equilibrium constant for R- and S-verapamil at their high affinity site on HSA.