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Assay signal vs time. Time-lapse uncompressed AVI files were acquired (1 frame per 30 s) and were analyzed using ImageJ (n = 4, error bars = s.d.). The large error bars between 25 and 50 min are due to variability in the time at which enhancement began, rather than the rate of enhancement. Over 3-fold enhancement is achieved through the use of the gold enhancement system.
