Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cancer Immunol Res. 2014 Jun 18;2(7):616–631. doi: 10.1158/2326-6066.CIR-14-0027

Figure 4. Gene expression signatures in lymphoid aggregates associated with immune responses and overall survival.

Figure 4

Gene expression measured by microarray was compared between microdissected lymphoid aggregates from 14 tumors grouped according to overall patient survival (OS > 3 years vs. < 1.5 years), post-vaccination induction of enhanced mesothelin-specific T-cell responses in PBL (Enh vs. Unenh), and the intratumoral CD8+ T effector/FoxP3+ Treg ratio (High vs. Low CD8/Foxp3 ratio). Gene ontology analysis revealed the enrichment of differentially expressed genes encoding numerous chemokine and chemokine receptor, integrin and adhesion molecule, NFkB pathway, and UPS components (Figure S6). Differences in Treg and Th17 pathway but not the Th1 or Th2 pathway genes were also associated with improved post-vaccination responses and survival (Figure S7). The linear fold-change in expression for each comparison is shown for representative genes in: (A) Chemokine, chemokine receptor, and integrin genes; (B) NFKB pathway genes; (C) T-helper pathway genes; and (D) PD-1 and PD-L1.