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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Photochem Photobiol. 2014 Apr 1;90(4):889–895. doi: 10.1111/php.12270

Figure 7.

Figure 7

Effects of Bafilomycin on PDT-induced hydroxyl radical formation. Cultures were incubated for 60 min with 2.5 μM APF and (as specified) 50 μM Baf, 0.5 μM BPD, or 40 μM NPe6 before being washed and refed. Treatments were: (A) untreated control, (B) Baf alone, (C) low-dose BPD PDT, (D) low-dose NPe6 PDT, (E) NPe6 PDT → BPD PDT, (F) Baf → BPD PDT. Panel (G) shows the pixel brightness analyses of 100 cells per treatment. *Significantly greater fluorescence than in untreated controls, BAF alone, and low dose NPe6 PDT, p<0.05. †Significantly greater than all other treatment groups, p< 0.05.