Figure 11.

A bona fide sense/antisense gene with amino acid activation activity expressed from both strands. A. Sequence and structural homology of the Class I HIGH signature and the F1 ATPase Walker A sequence. Glycine residues occur in exactly the same locations. The difference between the two sequences is that the Class I signature is specific for the α-phosphate group, whereas the Walker A signature is specific for the β-phosphate group. B. A designed sense/antisense gene coding for the 46-residue Class I aaRS and Class II ATP binding sites on opposite strands. C. Time course of leucine activation by the Class I 46mer. D. Time course of histidine activation by the Class II 46mer. Both plots have data for two concentrations of amino acid (10, 32 mM) and peptide (0.44, 1.75 μM). Scatter within each set of points is very noisy, so statistical analyses of various dependences are given in Table 3.