Table 1.
Cell cycle analysis of K-562 tumor cell lines after treatment with cis -tetraammine(oxalato)ruthenium(III) dithionate
| Control | Ru 1.5 μM | Ru 10 μM | Ru 40 μM | Ru 150 μM | |
|---|---|---|---|---|---|
| 24 h | Mean ± S.D. | Mean ± S.D | Mean ± S.D | Mean ± S.D | Mean ± S.D |
| Sub-G0/G1 | 14.45 ± 0.6 | 30.20 ± 2.1 | 31.20 ± 1.4 | 59.00 ± 2.3 | 68.90 ± 1.8 |
| G1 | 27.20 ± 0.3 | 25.25 ± 2.1 | 19.55 ± 0.9 | 11.50 ± 1.7 | 14.75 ± 0.9 |
| S | 19.05 ± 0.1 | 27.50 ± 1.1 | 29.10 ± 1.1 | 18.60 ± 2.1 | 11.40 ± 0.8 |
| G2 | 14.10 ± 0.1 | 14.85 ± 0.8 | 18.10 ± 0.7 | 9.15 ± 1.6 | 4.00 ± 0.1 |
| 48 h | |||||
| Sub-G0/G1 | 10.70 ± 0.7 | 24.65 ± 1.1 | 40.55 ± 2.6 | 68.60 ± 1.1 | 67.60 ± 0.1 |
| G1 | 35.65 ± 0.6 | 31.70 ± 1.0 | 14.85 ± 2.6 | 8.35 ± 0.4 | 19.30 ± 0.4 |
| S | 21.35 ± 0.4 | 30.05 ± 0.8 | 26.35 ± 2.1 | 14.35 ± 0.9 | 9.75 ± 0.2 |
| G2 | 10.00 ± 1.4 | 12.50 ± 0.3 | 17.05 ± 2.3 | 7.85 ± 0.1 | 2.95 ± 0.1 |
| 72 h | |||||
| Sub-G0/G1 | 15.50 ± 16.0 | 34.50 ± 1.4 | 45.95 ± 1.1 | 84.70 ± 0.3 | 70.50 ± 2.8 |
| G1 | 35.45 ± 10.1 | 31.75 ± 0.5 | 17.05 ± 0.2 | 4.95 ± 0.1 | 18.70 ± 1.0 |
| S | 20.05 ± 4.7 | 23.80 ± 0.6 | 26.05 ± 0.6 | 7.20 ± 0.6 | 9.25 ± 1.6 |
| G2 | 7.45 ± 0.8 | 9.30 ± 0.4 | 10.50 ± 0.3 | 3.10 ± 0.1 | 1.45 ± 0.2 |
The percentage of cells in G0/G1, S, G2/M and sub-G1 was analyzed using ModFit LT software (Verity Software House, Topsham, ME, USA). Percentage of viable cells at each phase of the cell cycle (G0/G1, S, G2/M). Data represent mean values ± SD of triplicate samples or a histogram for each condition and cell type. The results shown are representative of at least three similar experiments. *p < 0.05 vs. control.