Fig. 3.

CCL5 secreted by old quiescent NHFs stimulates MB231 breast cancer cell proliferation. a MB231 cells were cultured in presences of different amounts of recombinant human CCL5 and cell number counted after 6 days of the addition of CCL5. Asterisks represent statistical significance compared to cell numbers in untreated control cultures; n = 3, p < 0.05. b Conditioned media treated with anti-human CCL5 monoclonal antibody (0.4 μg/ml) was used to culture MB231 cells and cell number counted at 6 days after plating. Asterisks represent statistical significance compared to cultures grown in conditioned media collected from young quiescent NHFs; n = 3, p < 0.05