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CD95 induces cyt c release in a Bcl-2– and FLIPL-inhibitable manner. Cytosolic extracts (10 μg of protein) of J neo and J Bcl-2 (a), and J16 and JFL2 (b), prepared after the indicated times of treatment with anti-CD95 mAb (200 ng/ml), were separated by SDS-PAGE, and cyt c and actin content were evaluated by immunoblotting.
