Figure 5.

Bcl-2 abrogates Cer formation induced by etoposide or IR and reduces CD95-induced Cer formation. (a) Jurkat cells transfected with vector (J neo; filled bars) or with human bcl-2 cDNA (J Bcl-2; hatched bars) labeled with [¹⁴C]serine were exposed to etoposide (10 μg/ml) or IR (30 Gy). Cer levels were determined after 14 and 18 h and expressed as -fold increase (means ± SD) relative to Cer levels in time-matched untreated cells. (b) J Neo or J Bcl-2 cells labeled with [¹⁴C]serine were treated with anti-CD95 mAb (200 ng/ml). Cer formation and nuclear segmentation were determined in the same set of samples at the indicated times. Solid lines show Cer expressed as -fold increase relative to control cells and represent three independent experiments. Dotted lines show the percentage of nuclear segmentation.