Fig. 4.

Age prolongs cardiomyocyte Ca²⁺ transient decay in AngII-TG female mice. Cardiomyocytes were loaded with 1-μM Fura2 Ca²⁺ dye to evaluate Ca²⁺ cycling. a Ca²⁺ transient time constant of decay (ms), expressed as a percentage of young adult WT for each sex. b Representative cardiomyocyte Ca²⁺ transient profiles for young and aged adult male AngII-TG mice. c Representative cardiomyocyte Ca²⁺ transient profiles for young and aged adult female AngII-TG mice. Traces have been normalised to match the amplitude to enable direct time course comparisons n = 9–11 cells/group. Data are presented as mean ± SEM. *p(genotype) < 0.05, p(interaction) < 0.05, two-way ANOVA