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. 2014 Jun 19;15(1):496. doi: 10.1186/1471-2164-15-496

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© Zysset-Burri et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparative 2D gel electrophoresis of highly (A and C) and weakly (B and D) pathogenic N. fowleri. The proteomes of trophozoites were separated via 2D gel electrophoresis, and differentially expressed proteins (squares) were excised and identified via nano-liquid chromatography tandem mass spectrometry (nano-LC MS/MS). In the figure, only enlarged images from gel segments with differential spot patterns are shown. The numbers correspond to the identified proteins listed in Table 3. Spot 5 was used as a control, representing a protein (cofilin) with equivalent expression in highly and weakly pathogenic trophozoites.