Table 3.

Proteins that were differentially expressed in highly and weakly pathogenic N. fowleri, as identified via 2D gel electrophoresis in combination with nano-LC MS/MS

Spot no	Accession	Protein description	Species	Mol. weight	Theoretical pI	Regulation
1	AY684788	Heat shock protein 70 (hsp70)	N. fowleri	71408 Da	5.14	up
2	M90311	Actin 1	N. fowleri	41728 Da	5.26	up
3	M90312	Actin 2	N. fowleri	41153 Da	5.23	up
4	XM_002673759	Hsp20 domain containing protein	N. gruberi	19836 Da	6.20	down
5	XM_002669269	Cofilin (control)	N. gruberi	15384 Da	5.90	equal
6	XM_002681214	Cyclophilin	N. gruberi	19333 Da	6.29	up
7	AY049749	Membrane protein Mp2CL5	N. fowleri	19932 Da	6.82	up

The molecular weight and theoretical pI values of the identified proteins were calculated according to the Compute pI/Mw tool from the SIB Bioinformatics Resource Portal (http://web.expasy.org/compute_pi/). The results of visual quantification of the protein concentrations found in highly pathogenic compared to weakly pathogenic trophozoites are indicated in the last column (Regulation).