Table 4.
Differentially expressed proteins in highly and weakly pathogenic N. fowleri, as identified via 1D gel electrophoresis in combination with nano-LC MS/MS
| Accession Swissport | Hit Definition | Species | NP: HP/WP | PLP: HP/WP |
|---|---|---|---|---|
| Q3SZP7 | Villin-1 | Bos taurus | 16.87 | 10.19 |
| Q55585 | Probable succinate-semialdehyde dehydrogenase [NADP (+)] | Synechocystis sp. PCC 6803 substr. Kazusa | 9.09 | 11.63 |
| Q4UB16 | Ras-related protein Rab-1 | Theileria annulata | 8.88 | 9.39 |
| P08799 | Myosin II heavy chain | Dictyostelium discoideum | 8.03 | 3.86 |
| Q4R550 | Cysteine--tRNA ligase, cytoplasmic | Macaca fascicularis | 7.35 | 4.79 |
| B7MMS8 | Gamma-aminobutyraldehyde dehydrogenase | Escherichia coli S88 | 7.17 | 7.48 |
| O75382 | Tripartite motif-containing protein 3 | Homo sapiens | 6.43 | 3.91 |
| Q9V4N3 | Cytochrome b5; Short = CYTB5 | Drosophila melanogaster | 4.46 | 2.62 |
| P27420 | Heat shock 70 kDa protein C | Caenorhabditis elegans | 4.14 | 2.77 |
| Q9N1T2 | X-linked retinitis pigmentosa GTPase regulator | Canis lupus familiaris | 4.12 | 4.97 |
| D2VAA9 | Methylthioribose-1-phosphate isomerase | Naegleria gruberi | 3.91 | 2.90 |
| Q25544 | 26S protease regulatory subunit 8 homolog | Naegleria fowleri | 3.88 | 2.50 |
| P54772 | Histidine decarboxylase | Solanum lycopersicum | 2.91 | 8.22 |
| P10733 | Severin | Dictyostelium discoideum | 2.88 | 2.73 |
| Q1HPW4 | Eukaryotic translation initiation factor 3 subunit I | Bombyx mori | 2.85 | 2.23 |
| Q54BW4 | Circularly permutated Ras protein 2 | Dictyostelium discoideum | 2.46 | 3.79 |
| Q12965 | Unconventional myosin-Ie | Homo sapiens | 2.30 | 3.56 |
| P34552 | Apoptosis-linked gene 2-interacting protein X 1 | Caenorhabditis elegans | 2.30 | 2.21 |
| Q54K50 | Phospholipase D Y | Dictyostelium discoideum | 2.22 | 2.19 |
| Q5TJ55 | Formin-D | Dictyostelium discoideum | 2.18 | 8.77 |
| Q1ZXF7 | GDP-mannose 4,6 dehydratase | Dictyostelium discoideum | 2.15 | 2.44 |
| Q8IV36 | Protein HID1 | Homo sapiens | 2.07 | 2.71 |
| P13629 | Periplasmic [Fe] hydrogenase large subunit | Desulfovibrio oxamicus (strain Monticello) | −2.14 | −2.86 |
| P08964 | Myosin-1 | Saccharomyces cerevisiae S288c | −2.25 | −5.91 |
| P51824 | ADP-ribosylation factor 1 | Solanum tuberosum | −2.38 | −6.44 |
| A7HBL7 | Elongation factor Tu | Anaeromyxobacter sp. Fw109-5 | −2.46 | −2.42 |
| Q9CR62 | Mitochondrial 2-oxoglutarate/malate carrier protein | Mus musculus | −2.56 | −3.14 |
| Q1ZXF1 | Probable enoyl-CoA hydratase, mitochondrial | Dictyostelium discoideum | −2.69 | −4.44 |
| F4P6T0 | Ubiquinol oxidase, mitochondrial | Batrachochytrium dendrobatidis JAM81 | −2.97 | −5.61 |
| P77735 | Uncharacterized oxidoreductase YajO | Escherichia coli K-12 | −3.06 | −2.15 |
| Q889U1 | Single-stranded DNA-binding protein | Pseudomonas syringae pv. tomato str. DC3000 | −3.19 | −3.39 |
| P48375 | 12 kDa FK506-binding protein | Drosophila melanogaster | −3.55 | −2.95 |
| Q2G8K9 | Elongation factor Ts | Novosphingobium aromaticivorans DSM 12444 | −3.56 | −2.27 |
| Q7S6S4 | Mitochondrial import inner membrane translocase subunit tim-16 | Neurospora crassa OR74A | −3.67 | −2.80 |
| P54168 | Uncharacterized protein YpgQ | Bacillus subtilis subsp. subtilis str. 168 | −3.9 | −3.05 |
| O42899 | Protein sco1 | Schizosaccharomyces pombe 972 h- | −3.91 | −7.12 |
| Q97FZ9 | Rubrerythrin-1 | Clostridium acetobutylicum ATCC 824 | −4.94 | −2.06 |
| Q9P7M0 | ABC1 family protein C21C3.03, mitochondrial | Schizosaccharomyces pombe 972 h- | −5.73 | −2.62 |
| P42730 | Chaperone protein ClpB1 | Arabidopsis thaliana | −6.29 | −4.14 |
| P54872 | Hydroxymethylglutaryl-CoA synthase A | Dictyostelium discoideum | −6.3 | −3.2 |
| Q60597 | 2-oxoglutarate dehydrogenase, mitochondrial | Mus musculus | −8.13 | −2.87 |
| P42125 | Enoyl-CoA delta isomerase 1, mitochondrial | Mus musculus | −8.93 | −2.44 |
| O67589 | Aspartate--tRNA ligase | Aquifex aeolicus VF5 | −20.48 | −11.26 |
The proteins are sorted according to their decreasing level of up- or down-regulation in reference to weakly pathogenic N. fowleri. The regulation levels are given in columns 4 (NP: HP/WP: highly pathogenic trophozoites in Nelson’s medium versus weakly pathogenic trophozoites in PYNFH medium) and 5 (PLP: HP/WP: highly pathogenic trophozoites in PYNFH/LH medium versus weakly pathogenic trophozoites in PYNFH medium). The 22 up-regulated proteins (HP/WP > 2) were considered to be potential pathogenicity factors of N. fowleri.