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Northern analysis of poly(A)+ RNA samples prepared from implantation sites obtained at E9 and E11, placenta at E15, and pooled samples of uterus at E13 and E15. Samples were probed with a rat D3 cDNA. The amounts of poly(A)+ RNA loaded in each lane were 40, 31, 33, and 27 μg, respectively, from left to right. After hybridization and washing, the blot was subjected to autoradiography for 20 h.
