Figure 5.

In situ hybridization using the D3 antisense probe on sections of a uterine implantation site on approximate day E12–E13. (a) Low-powered photomicrograph of a longitudinal section showing the antimesometrial portion of the implantation site. Arrows point to specific signal from the D3 antisense probe that is present in the epithelial cells lining the uterine lumen lateral to the implantation site. Signal is also seen in the epithelial cells lining the recanalized uterine lumen (arrowheads) that abuts the fetal cavity. (b) A higher-power dark-field photomicrograph of the region shown in the box in a, demonstrating signal over the epithelial cells lining the uterine lumen. (c) A higher-power light-field photomicrograph, taken from a different specimen, of a cross-section of the uterine lumen in the region of the diagonal line shown in a. Silver grains are apparent over the epithelial cells lining the uterine cavity. (d) A dark-field photomicrograph of the same section shown in c. DB, decidual basalis; DC, decidual capsularis; FC, fetal cavity; P, placenta; RUL, recanalized uterine lumen; UL, uterine lumen.