Figure 6.

Plasma clearance of ¹²⁵I-labeled VLDL in wild-type (WT), TgBP-1a, LDLR^–/–, and TgBP- 1a;LDLR^–/– mice. VLDL was isolated from four fasted male TgBP-1a;LDLR^–/– mice and labeled with ¹²⁵I as described in Methods. ¹²⁵I-labeled VLDL (15 μg protein, 860 cpm/ng apo B protein) was injected intravenously into each of five 12-week-old male mice with the indicated genotype. Blood was drawn at the indicated times to quantify the content of ¹²⁵I-labeled apo B in plasma. (a and b) Plasma content of ¹²⁵I-labeled total apo B was measured by isopropanol precipitation followed by scintillation counting (as described in Methods). (c and d) Plasma samples were subjected to SDS-PAGE, and the signal generated by apo B-48 was quantified using a Fuji PhosphorImager as described in Methods. The data are plotted as the percent of the zero time value. *P < 0.05 (Student’s t test) compared with wild-type values. Each value represents the mean ± SEM of data from five mice.