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. 2014 May 13;11:9. doi: 10.1186/1742-4933-11-9

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Copyright © 2014 Lanzer et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Thymectomy reduces the response of NP-specific CD4 T cells in the lung airways of influenza virus-infected mice. Young mice, aged mice (19–20 months old) and thymectomized and sham thymectomized 10 month old mice (9 months after thymectomy) were infected with 3,000 EID₅₀ ×-31 influenza virus and the bronchoalveolar lavage (BAL) was assessed at 10 and 12 days post infection for total CD4 T cells and Flu NP-specific CD4 T cells. The absolute number of NP_311–325/IA^b-specific CD4 T cells in the BAL of individual mice from a representative experiment of two is shown. Data were analyzed by unpaired t-test with Welch correction (**, p < 0.005; NS, not significant).