Figure 1.

Class C and D Vps proteins requirements for efficient transcription of the Gat1-activated DAL5 gene following rapamycin treatment or transferring cells from ammonia to proline medium in YNB-grown cells. Total RNA was isolated from WT (TB50), gln3Δ (FV005), gat1Δ (FV006), the Class C pep3Δ (MK46), pep5Δ (MK24), and vps16Δ (FV735) and the Class D vps3Δ (MK23), vps34Δ (FV391), and vps45Δ (FV643) mutant cells grown in YNB-ammonia medium (Am.) and treated with rapamycin (+Rap) or transferred to proline medium (shift Pro). DAL5 mRNA levels were quantified by quantitative RT-PCR as described in “Experimental Procedures.” The values reported represent the averages of at least two experiments from independent cultures; error bars indicate standard errors.