Figure 2.

Sucrose gradient centrifugation of detergent-solubilized membranes from E. coli strains containing a FP fusion on sdhC. The succinate/ferricyanide oxidoreductase activity (▪) and the fluorescence of mCherry (□) were measured in extracts from strains BW25113 (A) and BW25113 mcherry-sdhC (B). All data were normalized to 10 mg membrane protein extract applied per gradient. The fluorescence of mCherry was measured at an excitation wavelength of 587 nm and an emission wavelength of 610 nm.