Table 1.
Characterization of bacterial isolates from Baltic microalgae Nitzschia microcephala, Isochrysis sp., Chlorella pyrenoidosa, and Scenedesmus obliquus
| 16S rDNA analysis | Antibiotic susceptibility (antibiotic μg/disk) | High similarity with | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Bacteriacode | Morphology | Highest BLAST hit | % highest BLAST hit | Gentamycin 50 | Streptomycin 25 | Kanamycin 25 | Rifampicin 5 | Meropenem 5 | Ticarcillin 50 | Cefotaxime 50 | Ampicillin 50 | |
| Nitzschia microcephala | ||||||||||||
| N11 | Slender rod; beige | Loktanella sp. | 100 | 1 | 1.8 | – | 4.1 | 1.8 | 4.1 | 5 | 3.3 | N2, N4, N7 |
| N2 | Slender rod; beige | Loktanella sp. | 100 | 1.5 | 1.8 | – | 4.1 | 1.8 | 4 | 5 | 3 | N1, N4, N7 |
| N31 | Slender rod; beige | Loktanella sp. | 99 | – | 1.6 | – | 42 | 2 | 4.2 | 5 | 3 | N53 |
| N4 | Slender rod; beige | Loktanella sp. | 99 | 1.5 | 1.7 | – | 4.1 | 2.1 | 4.5 | >5 | 2.6 | N1, N2, N7 |
| N51 | Slender rod; beige | Loktanella sp. | 100 | – | 1.5 | – | 4 | 2.2 | 3.7 | 4.7 | 3.9 | N33 |
| N61 | Plump pleomorphic rod; beige | Loktanella sp. | 99 | 2.4 | 1.5 | – | 4.02 | 3.3 | 3.6 | 5 | 2.6 | N9 |
| N7 | Slender rod; beige | Loktanella sp. | 100 | 1.5 | 2 | – | 3.82 | 2 | 3.8 | 5 | 3 | N1, N2, N4 |
| N81 | Short rod; beige | Loktanella sp. | 98 | – | 1.6 | – | 4 | 1.7 | 3.9 | 5 | 2.8 | |
| N9 | Plump pleomorphic rod; beige | Agrobacterium sp. | 99 | 32 | 1.6 | – | 4.2 | 2.1 | 4 | 5 | 3 | N6 |
| N101 | Very short rod, beige | Loktanella sp. | 100 | – | 1.5 | – | 2.6 | 1.5 | 2.8 | 5 | – | |
| Isochrysis sp. | ||||||||||||
| I11 | Pale slender rod; rust/orange | Flexibacter sp. | 95 | – | – | – | – | 1.4 | 2.5 | 1.5 | – | I2 |
| I2 | Pale slender rod; rust/orange | Flexibacter sp. | 100 | – | – | – | – | 1.4 | 2.3 | 1.5 | – | I1 |
| I31 | Short rod; light pink | Rhodobacteraceae bacterium JAM-ALO110 | 99 | 3.8 | 2.2 | – | 3.5 | 2.9 | 4.5 | >5 | 4.4 | |
| I41 | Large pleomorphic rod; cream | Seohaeicola saemankumensis SD-15 | 98 | 3.72 | 2.52 | 1.5 | 4 | 3.82 | 5 | >5 | 4.3 | |
| I51 | Large pleomorphic rod; cream | Roseobacter sp. | 99 | 3.72 | 2 | 1.7 | 4.5 | 4.5 | >5 | >5 | >5 | |
| I61 | Rods with cysts; cream | Flexibacter sp. | 99 | 3 | 2 | 1.4 | 4 | 3.5 | 5 | >5 | 4 | |
| I71 | Short rod, red/orange | Erythromicrobium sp. B04 | 100 | 2.4 | – | – | 4.7 | 4 | 4.5 | >5 | 3 | I8, I9, I10 |
| I8 | Short rod, red/orange | Erythromicrobium sp. B04 | 100 | 2.3 | – | – | 4.5 | 3.7 | 4.5 | >5 | 2.5 | I7, I9, I10 |
| I9 | Short rod, red/orange | Erythromicrobium sp. B04 | 99 | 2.3 | – | – | 4.5 | 4.3 | 4.5 | >5 | 3 | I7, I8, I10 |
| I10 | Short rod, red/orange | Erythromicrobium sp. B04 | 100 | 2.2 | – | – | 4 | 4 | 4.5 | >5 | 2.8 | I7, I8, I9 |
| Chlorella pyrenoidosa | ||||||||||||
| C11 | Short rod; pale | Agrobacterium vitis M63038 | 99 | 3.4 | 2.5 | 2 | >4 | >4 | >5 | >5 | 4.5 | C3, C9, S8 |
| C21 | Short rod; pale | Agrobacterium vitis M63038 | 96 | 3.5 | 2.4 | 1.6 | 3.6 | >4 | >5 | >5 | 4 | |
| C3 | Short rod; pale | Loktanella vestfoldensis IMCC6033 | 100 | 3.4 | 2.2 | 1.6 | 3.4 | 4 | >4.5 | 4.5 | 4.2 | C1, C9, S8 |
| C41 | Plump pleomorphic rod; pale | Loktanella vestfoldensis IMCC6033 | 99 | 2.8 | 1.5 | – | 4 | 3.2 | 3.8 | >5 | 2.7 | C6, C10 |
| C51 | Plump pleomorphic rod; pale | Agrobacterium vitis M63038 | 98 | 2.5 | 1.4 | – | 4 | 3.6 | 4 | >5 | 3.2 | C73, C83 |
| C6 | Plump pleomorphic rod; pale | Loktanella vestfoldensis IMCC6033 | 100 | 2.5 | 1.4 | – | 4.32 | 3.5 | 4 | >5 | 3.5 | C4, C10 |
| C71 | Plump pleomorphic rod; pale | Agrobacterium vitis M63038 | 98 | 3 | 1.5 | – | 4.3 | 3.5 | 3.6 | >52 | 2.5 | C53, C83 |
| C81 | Plump pleomorphic rod; pale | Agrobacterium vitis M63038 | 98 | 2.7 | 1.6 | – | 4.5 | 3.2 | 4 | >5 | 2.7 | C53, C73 |
| C9 | Short rod; pale | Loktanella vestfoldensis IMCC6033 | 100 | 3.4 | 2.1 | 1.7 | 3.5 | 4 | 5 | 4.4 | 3.7 | C1, C3, S8 |
| C10 | Plump pleomorphic rod; pale | Loktanella vestfoldensis IMCC6033 | 100 | 2.6 | 1.4 | – | 4.1 | 3.5 | 4 | 5.1 | 3.2 | C4, C6 |
| Scenedesmus obliquus | ||||||||||||
| S11 | Short rod; pale | Agrobacterium vitis M63038 | 98 | 3 | 2.2 | 1.5 | 3.2 | 2.64 | 5 | 4.4 | 4 | S2 |
| S2 | Short rod; pale | Agrobacterium vitis M63038 | 98 | 3.1 | 2.2 | 1.5 | 3 | 4 | 5 | 4.5 | 3.6 | S1 |
| S31 | Slender rod; beige | Rhizobium sp. W7 | 98 | 2.6 | 1.5 | – | 4.3 | 3.5 | 4 | 54 | 34 | S4, S6, S9, S10 |
| S4 | Slender rod; beige | Loktanella vestfoldensis IMCC6033 | 100 | 2.6 | 1.5 | – | 4 | 3.4 | 4.2 | 5.3 | 3.24 | S3, S6, S9, S10 |
| S51 | Short rod; pale | Loktanella vestfoldensis IMCC6033 | 100 | 2.8 | 1.5 | 2.42 | 3.2 | 3.64 | >5 | 4.2 | 3.7 | S73 |
| S6 | Slender rod; beige | Loktanella vestfoldensis IMCC6033 | 99 | 2.8 | 1.5 | – | 4.22 | 34 | 4.2 | 54 | 34 | S3, S4, S9, S10 |
| S71 | Short rod; pale | Loktanella vestfoldensis IMCC6033 | 100 | 3.6 | 3 | 2 | 3 | 3.54 | >5 | 4.2 | 3.5 | S53 |
| S8 | Short rod; pale | Loktanella vestfoldensis IMCC6033 | 100 | 3.3 | 2.5 | 2.82 | 3.2 | 3.84 | >5 | 4.4 | 4 | C1, C3, C9 |
| S9 | Slender rod; beige | Agrobacterium vitis M63038 | 98 | 3 | 1.6 | – | 4.4 | 44 | 4.7 | 5.5 | 3.5 | S3, S4, S6, S10 |
| S10 | Slender rod; beige | Loktanella vestfoldensis IMCC6033 | 100 | 3 | 1.6 | – | 4.54 | 44 | 5 | 5.5 | 3.8 | S3, S4, S6, S9 |
BLAST, Basic Local Alignment Search Tool.
1
Considered unique and used for the tree and UniFrac analysis.
2
Single colonies in inhibition zone.
3
Result of the maximum likelihood tree.
4
Inhibition zones were not totally clear.