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. 2014 May 9;3(3):383–394. doi: 10.1002/mbo3.177

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© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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ptk1 is required for maximal Porphyromonas gingivalis–Streptococcus gordonii community development. (A) CSLM visualization of P. gingivalis 33277, Δptk1, and Δptk1 + pptk1 (green) reacted with a substrate of S. gordonii (red) for 18 h. A series of 0.2 μm-deep optical fluorescent x–y sections (213 × 213 μm) were collected using an Olympus FV500 confocal microscope, digitally reconstructed into a 3D image and quantitated using Volocity software. (B) Total P. gingivalis biovolume was obtained with the 3D Find Objects function of Volocity. (C) Numbers of microcolonies using an object size cut-off algorithm of greater than 30 μm for the total 3D volume for P. gingivalis fluorescence. Error bars are SD, n = 3. ***P < 0.001 compared to Δptk1.