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. 2014 May 9;3(3):383–394. doi: 10.1002/mbo3.177

Figure 6.

Figure 6

Exopolysaccharide production by P. gingivalis is reduced in mutants lacking ptk1. (A) Exoploysaccharide produced by P. gingivalis 33277 strains was stained with FITC-labeled concanavalin A and wheat germ agglutinin. Bacterial cells were stained with Syto-17. Fluorescent images were collected by confocal microscopy. (B) Ratio of lectin binding (green) to whole cell staining (red) from images in (A) using Volocity software. Error bars are SD, n = 3. **P < 0.01; ***P < 0.001 compared to Δptk1 (C) Transmission electron microscopy analysis of P. gingivalis W83 wild type and ptk1 deficient mutant. Ruthenium red staining was employed to visualize capsule. Arrows indicate presence of surface capsule in W83 versus reduced capsule in Δptk1. Magnification, ×27,500. Scale bar = 0.5 μm.