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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Cell Stem Cell. 2014 May 8;15(1):92–101. doi: 10.1016/j.stem.2014.04.002

Fig. 2. OCT4 and SFRS2 display interdependent functional links in hPSC.

Fig. 2

(A–B) SFRS2 is required to support self-renewal. Lentiviral shRNA-mediated depletion of SFRS2 disrupted pluripotency in H1 ESC as monitored by (A) colony morphology and (B) expression of OCT4 and NANOG. (C) Depletion of OCT4 in H1 ESC for 2 and 5 days led to a coordinate decrease in the expression of SFRS2. (D) OCT4 selectively binds the proximal promoter region of SFRS2 in H1 ESC. (E) OCT4 depletion in human clone 9 iPSC disrupts luciferase expression downstream of the native SFRS2 promoter. (F) Mutation or deletion of the predicted OCT4 binding motif (ATGCCAAT) in the proximal SFRS2 promoter region decreased downstream luciferase expression in clone 9 iPSC. See also Fig. S4. Error bars represent the mean ± SEM.