Skip to main content
. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 11;193(2):663–672. doi: 10.4049/jimmunol.1302554

Figure 9. Attenuated EAE development in Id1 transgenic mice is associated with increased Treg counts and reduced encephalitogenic effector T cells.

Figure 9

(A) EAE was induced in 7-week-old WT and TG female littermates using MOG peptide and pertussis toxin. Disease progression was scored daily for 36 days using the criteria described in Materials and Methods. Results are presented as mean with SD. (B) The numbers of mononuclear cells harvested from the spinal cord after proteolytic digestion of the tissue are shown as mean with SD. (C) Expression of FoxP3 in draining lymph nodes and spinal cord was measured on day 14 after immunization and representative dot plots and average percentages of the indicated subsets are shown. (D) Examination of CD4+ effector T cells in draining lymph nodes and spinal cord. Cells were stimulated with PMA (50 ng/ml) and ionomycin (1 μg/ml) for 4 hours. IL-17A and IFNγ expression was detected using intracellular staining. Average percentages of the indicated subsets are shown.